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doxycycline dox inducible cas9 endonuclease  (Addgene inc)


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    Structured Review

    Addgene inc doxycycline dox inducible cas9 endonuclease
    Doxycycline Dox Inducible Cas9 Endonuclease, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dox+inducible+cas9/Lenti-iCas9-neo+(Plasmid+%2385400)/pm41337884-50-17-22
    Average 94 stars, based on 51 article reviews
    doxycycline dox inducible cas9 endonuclease - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression
    Article Snippet: .. To achieve p53 inactivation, cells were transducted with a lentiviral system consisting of Dox-inducible Cas9 (pCW-Cas9 was a gift from Eric Lander & David Sabatini, Addgene plasmid # 50661) and lentiguide for gRNA expression (lentiGuide-Puro was a gift from Feng Zhang, Addgene plasmid # 52963). ..

    Expressing:

    Article Title: IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression
    Article Snippet: .. To achieve p53 inactivation, cells were transducted with a lentiviral system consisting of Dox-inducible Cas9 (pCW-Cas9 was a gift from Eric Lander & David Sabatini, Addgene plasmid # 50661) and lentiguide for gRNA expression (lentiGuide-Puro was a gift from Feng Zhang, Addgene plasmid # 52963). ..

    Article Title: Sequencing-free whole genome spatial transcriptomics at molecular resolution in intact tissue
    Article Snippet: The backbone was modified from plasmid CROPseq-Guide-Puro (Addgene, 86708), and its sequence is included in File S1. .. Lentivirus was produced in HEK-293FT cells (Thermo Fisher Scientific, R70007) and was used to transduce an engineered A549 clone with dox-inducible Cas9 (Addgene, 83481) expression. .. Cells were under Puromycin (Thermo Fisher Scientific, A1113803) selection for one week until the cell library was established, and were always maintained in medium with Puromycin.

    Produced:

    Article Title: Sequencing-free whole genome spatial transcriptomics at molecular resolution in intact tissue
    Article Snippet: The backbone was modified from plasmid CROPseq-Guide-Puro (Addgene, 86708), and its sequence is included in File S1. .. Lentivirus was produced in HEK-293FT cells (Thermo Fisher Scientific, R70007) and was used to transduce an engineered A549 clone with dox-inducible Cas9 (Addgene, 83481) expression. .. Cells were under Puromycin (Thermo Fisher Scientific, A1113803) selection for one week until the cell library was established, and were always maintained in medium with Puromycin.

    Transduction:

    Article Title: Sequencing-free whole genome spatial transcriptomics at molecular resolution in intact tissue
    Article Snippet: The backbone was modified from plasmid CROPseq-Guide-Puro (Addgene, 86708), and its sequence is included in File S1. .. Lentivirus was produced in HEK-293FT cells (Thermo Fisher Scientific, R70007) and was used to transduce an engineered A549 clone with dox-inducible Cas9 (Addgene, 83481) expression. .. Cells were under Puromycin (Thermo Fisher Scientific, A1113803) selection for one week until the cell library was established, and were always maintained in medium with Puromycin.

    other:




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